Identification of pyrin targets by CHiP-Seq

Introduction Familial Mediterranean fever (FMF) is a recessive disorder characterized by episodes of fever and neutrophilmediated serosal inflammation. The gene causing FMF, MEFV, encodes a protein, pyrin. Pyrin is expressed predominantly in innate immune cells such as neutrophils, monocytes, and dendritic cells, but not in lymphocytes. Studies of pyrin localization show a cell-type dependency. Recent studies have demonstrated that the N-terminal fragment of cleaved pyrin binds to p65 and enhances its entrance into the nucleus. Also, we have previously shown by chromatin immunoprecipitation coupled with PCR (ChIP-qPCR) that in THP1 cells, pyrin can bind to the promoter of the transcription factor, IRF2.